Once your MySQL database has been setup, you can start adding information to it via our web based MySQL manager (PHPMyAdmin) through the control panel. Then you'll need to integrate it into your website. PHP, an easy to use powerful scripting language, is often utilized to interact with your MySQL database.
There are many great tutorials on combining PHP and MySQL for a great database-driven website. Here are a few:
Building a Database-Driven Web Site Using PHP and MySQL - Great tutorial covering all the basics.